Volume 6, Issue 4, December 2018, Page: 112-117
Hsa-miR-106b-5p Negatively Regulates LEF1
Annada Anil Joshi, Department of Biochemistry, T. N. Medical College & B.Y. L. Nair Ch. Hospital, Mumbai, India
Alka Vishwas Nerurkar, Department of Biochemistry, T. N. Medical College & B.Y. L. Nair Ch. Hospital, Mumbai, India
Neelam Vishwanath Shirsat, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, India
Received: Aug. 23, 2018;       Accepted: Sep. 10, 2018;       Published: Oct. 13, 2018
DOI: 10.11648/j.crj.20180604.11      View  244      Downloads  10
Abstract
Aberrant expression of the genes involved in Wnt signaling pathway, one of the most important developing pathways, is observed in many malignancies. Reports show that Wnt/β-catenin activation is critical for cancer development, angiogenesis, migration, and invasion. LEF1 belongs to the T cell Factor (TCF)/LEF family of transcription factors and plays the role of nuclear effector in the Wnt/β-catenin signaling pathway. LEF1 has central role as a transcription factor in the Wnt/β-catenin signaling pathway which makes it an ideal target for therapeutic treatment in dealing with cancer proliferation. It can act as an oncogene or a tumor suppressor in cellular context dependent manner. miRNAs are aberrantly expressed in cancers and can act as tumor suppressors or oncomirs depending upon the type of carcinomas. Studies show that miRNAs can be used as novel agents for targeted cancer therapy. miR-106b, which belong to miR-17-92 paralog cluster, is reported to be overexpressed in multiple tumor types including medulloblastomas, breast, colon, kidney, gastric, lung cancer and HCC. In this study we have demonstrated that over-expression of miR-106b-5p down-regulates the endogenous expression of LEF1 in HEK293FT cells, thereby affecting the expression of N-Myc, downstream gene of Wnt signaling. Therefore, our results suggest that miR-106b-5p plays a significant role in suppressing the carcinomas resulted due to the over-expression of LEF1 and/or activation of Wnt pathway and may prove to be a potential target for novel cancer therapy. It may helpful in developing therapeutic strategies for cancer treatments.
Keywords
WNT Signaling Pathway, LEF1, MYCN, Hsa-miR-106b, HEK293FT Cells, Western Blotting, Luciferase Assay
To cite this article
Annada Anil Joshi, Alka Vishwas Nerurkar, Neelam Vishwanath Shirsat, Hsa-miR-106b-5p Negatively Regulates LEF1, Cancer Research Journal. Vol. 6, No. 4, 2018, pp. 112-117. doi: 10.11648/j.crj.20180604.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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