Volume 6, Issue 3, September 2018, Page: 92-100
Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells
Hewida Hassan Fadel, Medical Laboratory Technology Department/ Faculty of Allied Medical Science, Pharos University in Alexandria, Alexandria, Egypt
Mohamed Ahmed Abdel Mohsen, Applied Medical Chemistry Department/ Medical Research Institute, University of Alexandria, Alexandria, Egypt
Khaled El Sayed Soliman, Experimental and Clinical Surgery Department/ Hospital of Medical Research Institute, University of Alexandria, Alexandria, Egypt
Hanaa Mohamed Kohail, Cancer Management and Research Department/ Hospital of Medical Research Institute, University of Alexandria, Alexandria, Egypt
Shehata Mahmoud EL Sewedy, Medical Laboratory Technology Department/ Faculty of Allied Medical Science, Pharos University in Alexandria, Alexandria, Egypt; Applied Medical Chemistry Department/ Medical Research Institute, University of Alexandria, Alexandria, Egypt
Received: Jun. 26, 2018;       Accepted: Jul. 9, 2018;       Published: Aug. 2, 2018
DOI: 10.11648/j.crj.20180603.14      View  396      Downloads  44
Abstract
In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (P<0.001) as well as ES (P<0.05). The serum level of CECs decreased significantly (P<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.
Keywords
Breast Cancer, Vitamins D3 and B6, Angiogenesis, VEGF, ES, Circulating Endothelial Cells
To cite this article
Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy, Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells, Cancer Research Journal. Vol. 6, No. 3, 2018, pp. 92-100. doi: 10.11648/j.crj.20180603.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Dey S, Soliman, AS, Hablas A, Seifeldein IA, Ismail K, Ramadan M, El-Hamzawy H, Wilson ML, Banerjee M, Boffetta P, Harford J, Merajver SD: Urban-rural differences in breast cancer incidence in Egypt (1999-2006). Breast 2010; 19(5): 417-23.
[2]
McPherson K, Steel CM, Dixon JM: Breast cancer, epidemiology, risk factors, and genetics. BMJ 2000; 321: 624-628.
[3]
Brown NS and Bicknell R: Hypoxia and oxidative stress in breast cancer Oxidative stress: its effects on the growth, metastatic potential and response to therapy of breast cancer. Breast Cancer Res 2001; 3: 323–327.
[4]
Kim YW and Byzova TV: Oxidative stress in angiogenesis and vascular disease. Blood 2014; 123 (5): 625-31.
[5]
Goon PKY, Lip GYH, Boos CJ, Stonelake PS, and Blann AD. Circulating Endothelial Cells, Endothelial Progenitor Cells, and Endothelial Microparticles in Cancer. Neoplasia 2006; 8(2): 79–88.
[6]
Bidard FC, Mathiot C, Degeorges A, Etienne-Grimaldi MC, Delva R, Pivot X, Veyret C, Bergougnoux L, de Cremoux P, Milano G and Pierga JY: Clinical value of circulating endothelial cells and circulating tumor cells in metastatic breast cancer patients treated first line with bevacizumab and chemotherapy. Ann Oncol 2010; 21: 1765-71.
[7]
Campbell MJ, Gombart AF, Kwok SH, Park S, Koeffler HP: The anti-proliferative effects of 1, 25 (OH) 2D3 on breast and prostate cancer cells are associated with induction of BRCA1 gene expression. Oncogene 2000; 19: 5091–7.
[8]
Cheng CH, Chang SJ, Lee BJ, Lin KL and Huang YC: Vitamin B6 supplementation increases immune responses in critically ill patients. Eur. J Clin. Nutr 2006; 60:1207-13.
[9]
Zhang XH, Ma J, Smith-Warner SA, Lee JE, Giovannucci E. Vitamin B6 and colorectal cancer: current evidence and future directions. World J Gastroenterol 2013; 19(7):1005-10.
[10]
Prieto P, Pineda M and Aguilar M: Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E. Analytical Biochemistry 1999; 269: 337-341.
[11]
Pendyala G, Thomas B and Kumari S: The challenge of antioxidants to free radicals in periodontitis. J Indian Soc. Periodontol 2008; 12: 79–83.
[12]
Tug T, Karatas F, Terzi SM, Ozdemir N. Comparison of Serum Malondialdehyde Levels Determined by Two Different Methods in Patients with COPD: HPLC or TBARS Methods. Laboratory Medicine 2005; 36: 41–44.
[13]
Byrne GJ, Mcdowell G, Agarawal R, Sinha G, Kumar S and Bundred NJ. Serum Vascular Endothelial Growth Factor in Breast Cancer. Anticancer Research 2007; 27: 3481-3488.
[14]
Sasaki T, Larsson H, Tisi D, Claesson-Welsh L, Hohenester E and Timpl R. Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity. J Mol. Biol. 2000; 301: 1179-1190.
[15]
Woywodt A, Blann AD, Kirsch T, Erdbruegger U, Banzet N, Haubitz M. Isolation and enumeration of circulating endothelial cells by immunomagnetic isolation: proposal of a definition and a consensus protocol. J Thromb. Haemost 2006; 4(3): 671-7.
[16]
D’Andrea GM. Use of Antioxidants During Chemotherapy and Radiotherapy Should Be Avoided. CA Cancer J Clin 2005; 55: 319–321.
[17]
Moss RW: Do Antioxidants Interfere With Radiation Therapy for Cancer? Integr Cancer Ther 2007; 6: 281-292.
[18]
Weijl NI, Elsendoorn TJ, Lentjes EG, Hopman GD, Wipkink-Bakker A, Zwinderman AH, Cleton FJ, Osanto S: Supplementation with antioxidant micronutrients and chemotherapy-induced toxicity in cancer patients treated with cisplatin-based chemotherapy: a randomised, double-blind, placebo-controlled study. Eur J Cancer 2004; 40:1713-23.
[19]
Slatter DA, Avery NC, Bailey AJ:Identification of a new cross-link and unique histidine adduct from bovine serum albumin incubated with malondialdehyde. J Biol. Chem 2004; 279: 61-9.
[20]
Zhao J, Yan F, Ju H, Tang J and Qin J. Correlation between serum vascular endothelial growth factor and endostatin levels in patients with breast cancer. Cancer Letters 2004; 204: 87–95.
[21]
Wu FP, Hoekman K, Meijer S, Cuesta MA: VEGF and endostatin levels in wound fluid and plasma after breast surgery. Angiogenesis 2003; 6(4): 255-7.
[22]
Conklin KA: Chemotherapy-Associated Oxidative Stress: Impact on Chemotherapeutic Effectiveness. Integrative Cancer Therapies 2004; 3: 294-300.
[23]
Nicolson GL. Lipid Replacement Therapy: A Nutraceutical Approach for Reducing Cancer-Associated Fatigue and the Adverse Effects of Cancer Therapy while Restoring Mitochondrial Function. Cancer and Metastasis Reviews 2010; 29(3): 543-552.
[24]
Areti A, Yerra VG, Naidu VGM and Kumar A: Oxidative stress and nerve damage: Role in chemotherapy induced peripheral neuropathy. Redox Biol 2014; 2: 289–295.
[25]
Shen J, Lai CQ, Mattei J, Ordovas JM, Tucker KL. Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study. Am J Clin. Nutr 2010; 91: 337–342.
[26]
Simone CB II, Simone NL, Simone V and Simone CB: Antioxidants and other nutrients do not interfere with chemotherapy or radiotherapy and can increase kill and increase survival part. Alternative therapies 2007; 13: 22-28.
[27]
Suwannalert P, Rattanachitthawat S, Chaiyasut C, and Riengrojpitak S: High levels of 25-hydroxyvitamin D3 [25(OH)D3] and α-tocopherol prevent oxidative stress in rats that consume Thai brown rice. Journal of Medicinal Plants Research 2010; 4(2):120-124.
[28]
Celik I, Surucu O, Dietz C, Heymach JV, Force J, Ho¨scheleI, Becker CM, Folkman J and Kisker O: Therapeutic Efficacy of Endostatin Exhibits a Biphasic Dose-Response Curve. Cancer Res2005; 65: 11044- 11050.
[29]
ChungI, Han G, Seshadri M. GillardBM, Yu WD, Barbara A. Foster BA, Trump DL and Johnson CS. Role of Vitamin D Receptor in the Antiproliferative Effects of Calcitriol in Tumor-Derived Endothelial Cells and Tumor Angiogenesis In vivo. Cancer Res 2009; 69(3): 967-975.
[30]
Rossi D and Catalano G: Pyridoxine as prophylactic therapy for palmar-plantar erythrodysesthesia associated with administration of pegylated liposomal doxorubicin (caelyx): a single-center experience. Oncology 2007; 73: 277-8.
[31]
Ben-Shoshan M, Amir S, Dang DT, Dang, LH, Weisman Y, Mabjeesh NJ: 1alpha, 25-dihydroxyvitamin D3 (Calcitriol) inhibits hypoxia-inducible factor-1/vascular endothelial growth factor pathway in human cancer cells. Mol. Cancer Ther 2007; 6:1433-9.
[32]
Grundmann M, Haidar M, Placzko S, Niendorf R, Darashchonak N, Hubel CA, von Versen-Höynck F: Vitamin D improves the angiogenic properties of endothelial progenitor cells. American Journal of Physiology - Cell Physiology2012; 303: 954-962.
[33]
Umeda T, Abe H, Cho H, Shimizu T, Mori T, Kubota Y, Kawai Y, Tanaka M, Kurumi Y, Tani T: An effective case of liver metastasis of breast cancer treated with capecitabine + docetaxel combination therapy using vitamin B6. GanTo Kagaku Ryoho 2010; 37: 687-9.
[34]
Lopes N, Paredes J, Costa JL, Ylstra B, Schmitt F. Vitamin D and the mammary gland: a review on its role in normal development and breast cancer. Breast Cancer Research 2012; 14(3):211-217.
[35]
Rugo HS. Bevacizumab in the Treatment of Breast Cancer: Rationale and Current Data. The Oncologist 2004; 9: 43–49.
[36]
Gröber U, Holzhauer P, Kisters K. Holick MF and Adamietz IA. Micronutrients in Oncological Intervention. Nutrients. 2016; 8(3): 163.
[37]
Ma Y, Trump DL and Johnson CS: Vitamin D in combination cancer treatment. J Cancer 2010; 1: 101-107.
[38]
Je Y, Lee JE, Ma J, Zhang X, Cho E, Rosner B, Selhub J, Fuchs CS, Meyerhardt J, and Giovannucci E:Prediagnostic plasma vitamin B6 (pyridoxal 5′-phosphate) and survival in patients with colorectal cancer. Cancer Causes Control 2013; 24(4):719-29.
[39]
Kuonen F, Secondini C, Rüegg C. Molecular pathways: emerging pathways mediating growth, invasion, and metastasis of tumors progressing in an irradiated microenvironment. Clinical Cancer Research 2012; 18(19): 5196-202.
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