Volume 6, Issue 2, June 2018, Page: 62-69
Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer
Moinul Abedin Shuvo, Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Chittagong, Bangladesh
Sayeedul Alam Prince, Institute of Nuclear Medicine and Allied Sciences, Bangladesh Atomic Energy Commission, Cox’s Bazar, Bangladesh
Arifuzzaman, Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Chittagong, Bangladesh
Received: Feb. 2, 2018;       Accepted: Feb. 25, 2018;       Published: Apr. 2, 2018
DOI: 10.11648/j.crj.20180602.14      View  727      Downloads  32
Abstract
Unlike almost all the cervical, penile, vulvar, and anal cancers, where Human papilloma virus has long known to play a vital role, a causative link between carcinogenic Human papilloma virus and lung cancer have been found to be highly variable and contradictory. Data also shows geography and race-dependenty. Apart from etiological factors, viral carcinogen can manipulate the cell cycle, hamper cell apoptosis and also interrupt the cell division in host cell which lead to the lung cancer. Molecular studies of carcinogenic Human papilloma virus have found that E6/E7 acts as mitotic mutators which play an important role in pathogenicity and oncogenicity. Analysis of genome sequence of Human papilloma virus revealed that ORF having conserved in early region, E6 and E7 required for viral pathogenicity and oncogenicity can be the suitable target for RNAi technology. RNAi works by silencing or turning off gene expression to control pathogenicity and oncogenicity by blocking its replication processes. Therefore, the work is done on the basis of rational siRNA designing method by targeting viral oncogenic E6 and E7 genes of Human papilloma virus types16 & 18. Searching siRNA target sequences, multiple sequence alignment, forecasting secondary structure and RNA-RNA interaction prediction was done by various computational software tools for designing RNA-based therapeutics (siRNA). In this study, four effective siRNA were predicted rationally for oncogenic E6 and E7 genes of Human papilloma virus types 16 & 18 which might be used as a potential RNA based therapeutics to control the rate of carcinogenesis and degree of oncogenicity. The outcome of this study provides a basis of the researchers towards understating to development of RNA-based therapeutics (siRNA) at genomic level.
Keywords
Lung Cancer, Human Papilloma Virus, RNA-Based Therapeutics, RNAi Technology
To cite this article
Moinul Abedin Shuvo, Sayeedul Alam Prince, Arifuzzaman, Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer, Cancer Research Journal. Vol. 6, No. 2, 2018, pp. 62-69. doi: 10.11648/j.crj.20180602.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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