Volume 6, Issue 2, June 2018, Page: 51-61
N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)
Adeleke G. E., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Adedosu O. T., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Oyewo E. B., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Fatoki J. O., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Abioye D. H., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Ishola A. A., Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Maduagwu E. N., Department of Biological Sciences, Biochemistry Unit, Covenant University, Ota, Nigeria
Received: Dec. 31, 2017;       Accepted: Mar. 5, 2018;       Published: Mar. 27, 2018
DOI: 10.11648/j.crj.20180602.13      View  432      Downloads  12
Abstract
This study investigated the nitrosating activities of intact cells and cell fractions of Bacillus cereus isolated from fermented palm wine (Elaeis guineensis) during incubation with dimethylamine and nitrite or nitrate. The pH values of the incubation mixtures were 7.2 ± 0.07 and 7.1 ± 0.07 in the presence of nitrite and nitrate, respectively. The N-Nitrosodimethylamine (NDMA) level in cell debris was significantly higher compared with cell extract, cell suspension and sterile controls. The time-course of NDMA formation in cell debris showed a significant (p < 0.05) early decline followed by an increase. The kinetic data obtained from Lineweaver-Burk plots of NDMA formation showed that Michaelis -Menten constant (Km) value was 37.5% lower, while initial velocity (Vmax) was 20.0% higher in cell debris relative to cell extract, when a fixed DMA concentration was used against varying nitrite concentrations. However, at a fixed nitrite concentration against varying DMA concentration, Km value was 44.4% lower, while Vmax was 166.7% higher in cell debris compared with cell extract. Investigation of nitrosation mechanism showed that Vmax values in the cell debris were 41.7, 50.0 and 55.6 μmol NDMA/mg protein, while the values were 34.5, 37.0 and 43.5 μmol NDMA/mg protein in cell extract for the respective three nitrite concentrations. This study has shown that intact Bacillus cereus cells catalyzed nitrosation of dimethylamine at near neutral pH, and the nitrosation, which was higher in cell debris than cell extract of the bacterium, followed a sequential mechanism of enzyme catalysis.
Keywords
Bacillus cereus, Dimethylamine, Enzyme Mechanism, Palm Wine, N-Nitrosation
To cite this article
Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., Ishola A. A., Maduagwu E. N., N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis), Cancer Research Journal. Vol. 6, No. 2, 2018, pp. 51-61. doi: 10.11648/j.crj.20180602.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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